Increasing salinity tolerance in tomato solanum lycopresicum l. The analysis focused on early response genes after severe salt stress in the root tissue of tomato seedlings. Proteomic analysis of saltstress responsive proteins in. Identification of early salt stress response genes in. At the molecular level, shortterm treatment with ej01 increased the expression of salt stress responsive genes such as dreb2b, rd29a, rd29b, and rab18 in arabidopsis. Loss of salt tolerance during tomato domestication. Slmyb102, an r2r3type myb gene, confers salt tolerance in. Physiological and genetic characterization of salt tolerance in tomato lycopersicon esculentum plant growth is limited by different environmental conditions. Upon salt stress, the expression of certain genes in the root was changed within 15 min kawasaki et al.
The object of the work is to evaluate whether rhizobacteria populating dry salty environments can increase resistance in tomato to salt stress. Durinta was grafted on five rootstocks being used commercially in the mediterranean. In addition, altered expression was observed for many other slwrky genes in tomato, including induction of slwrky23, slwrky33, and slwrky57 under salt stress huang et al. Pdf alleviation of salt stress in cucumber and tomato by. Nevertheless, most results have so far demonstrated that over. Salt induced soil degradation is common in farmlands and limits the growth and development of numerous crop plants in the world. Pdf effect of salt stress on expression of carotenoid. Seven strains of plant growthpromoting bacteria that have 1aminocyclopropane1carboxylate acc. Cytokinins cks are involved in response to various environmental cues, including salinity. Additionally drought stress during cultivation increased the antioxidant capacity of tomato fruit while maintaining the lycopene. Salt stress induction of proteinase inhibitor ii inh ii accumulation. Crops differ in their ability to grow under saline conditions and their responses are quite variable and not fully understood. Effect of salt stress on growth and chlorophyll content of.
It indicates the expression of functional gene occurs at high salt stress. Role of exogenous calcium article pdf available in journal of plant sciences 106. Therefore, salt stress seems to negatively affect tomato growth and would be expected to cause significant crop yield losses. The tomato slwrky3 gene, induced by diverse osmotic stresses and salicylic. Pdf salt stress is one of the most devastating abiotic stress which severely. Combined biotic and abiotic stress resistance in tomato.
Gene slcomt1 improves the resistance of tomato to salt stress. However it is possible to select callus line tolerant to elevated levels of nacl stress by sudden exposure to high of nacl, accordingly a nacl tolerant cell line was selected from hypocotyls and cotyledon derived callus of tomato which proved to be a true cell line variant. In the present study tomato plants were subjected to three different levels of salt stress to evaluate the effects of salt stress on antioxidant defence mechanisms in fruits. Differences in sensitivity towards salt stress led to the classification of 15 local genotypes out of 20 as sensitive classes 3, 4 and 5. Saline conditions are known to suppress the growth of crops. Perusal of the data show that the tested genotypes responded differently to nacl stress table 1 and table 2. A tomato bzip transcription factor, slareb, is involved in water deficit and salt stress response. Effect of salt stress on physiological response of tomato fruit grown. Rubpco activities, chloroplast area, and the number of plastoglobuli remained. Pdf application of chlorophyll fluorescence for the. The tomato ars1 altered response to salt stress 1 gene encodes a myb transcription factor that contributes to reduced transpiration water loss under salt stress, which is mediated by aba campos et al. However, for tomato crops, moderate salt stress improves the fruit quality, increasing nutritional components but decreasing fruit yield. Growth under salt stress was correlated with growth under non stress conditions, however the degree of correlation r 0. Furthermore, the relationship between melatonin production and salt resistance of transgenic tomato plants was investigated.
It is reported that about 7 % of the total earths land and 20 % of the total. Judged by both na and cl accumulations and maintenance of k, ca and mg contents with increasing salinity, the most tolerant cultivars pera and gc72 showed different responses. The functional biology of salt stress adaptation in plants is matter of current debate. Elimination of salt from the soil is time consuming and very expensive. The effect of water stress and salinity on growth and.
Salt stress is a rising threat to crop productivity, among several notable consumed crops tomato is often under threat due to salt stress. Effect of salt stress on expression of carotenoid pathway genes in tomato. Effect of salt stress on ion concentration, proline. Salt stress response in tomato beyond the salinity. The combined effects of salinity and water stress on the.
Grafting high yielding but salt susceptible tomato cultivars onto salt resistanttolerant rootstocks is a sustainable strategy to overcome saline stress. Salinity acclimation ameliorates salt stress in tomato. Salicylic acidinduced improvement of of salt stress. Response of two tomato culitvars differing in salt tolerance to inoculation with mycorrhizal fungi under. A randomized complete block design experiment with three treatments and four replications was conducted. Pdf plant growthpromoting bacteria confer resistance in. In this study, we isolated salt tolerant bacteria from the rhizosphere of tamarix chinensis, suaeda salsa and zoysia sinica, which are common wild plants grown on a salinealkaline land, to test these bacterias efficiency in alleviating salt stress in tomato plants.
Moreover, only a small number of genes playing important roles in tolerance mechanisms to drought andor salinity have been identified so far. It has been reported that exogenous application of aba reduces the release of ethylene and leaf abscission under salt stress in plants, probably by decreasing the accumulation of toxic clions in leaves gomez et al. Differential responses to shortterm salinity stress of heattolerant. The effects of two soil salinities 1gkg and 6gkg and two water stress levels 60% and 80% of the field capacity on growth and yield parameters of meiguo903 tomato were investigated under greenhouse conditions. It has been previously reported that enhancing ck contents improved salt stress tolerance in tomato.
One possibility to circumvent this problem is to use root colonizing, salt tolerant bacterial inoculants, which can alleviate salt stress in plants. Author links open overlay panel shimon mayak a tsipora tirosh a bernard r glick b. Fruit growth rate, water status, cuticle permeability and induction of blossomend rot ber of tomato fruit were considered for this study. Gene slcomt1 improves the resistance of tomato to salt. Effect of salt stress on the growth and fruit quality of. In vitro selection of calli for salt tolerance in tomato. A study was conducted using ten genetically diverse genotypes along with their 45f 1 generated by diallel mating under normal and salt stress conditions. Fruit quality, salinity stress, soilless cultivation, tomato growth, water use efficiency, yield. Salicylic acidinduced improvement of of salt stress resistance of tomato.
Effect of salt stress on tomato fruit antioxidant systems. Initial, and total activity and activation state state of rubpco were also measured. A tomato bzip transcription factor, slareb, is involved in. Transgene and mutation analyses have both substantially contributed to identify major salt tolerance determinants and to dissect the complexity of multiple mechanisms leading to stress adaptation chinnusamy et al. Response of the cultivated tomato and its wild salt. Plant growthpromoting bacteria confer resistance in tomato plants to salt stress.
The response of the antioxidant system to salt stress was studied in the roots of the cultivated tomato lycopersicon esculentum mill. Pdf salt stress response in tomato beyond the salinity. Salt stress activation of woundrelated genes in tomato plants. Salt stress is one of the major abiotic stresses limiting crop production especially in arid and semiarid regions. Plant growthpromoting bacteria confer resistance in. Rio grande to salinity, the effect on plant growth, water relations, stomatal conductance and chlorophyll fluorescence was investigated. Hsieh th1, li cw, su rc, cheng cp, sanjaya, tsai yc, chan mt. Plant growth is limited by different environmental conditions. The effect of foliarapplied glycinebetaine gb on chloroplast ultrastructure, the amount of chlorophyll and proteins and ribulose1,5bisphosphate carboxylaseoxygenase rubpco activity in stressed tomato leaves were analysed. Thirty tomato accessions representing six lycopersicon species were evaluated for the rate of seed germination under no stress, cold.
Effect of salt stress on growth attributes of ten genotypes of tomato in the present experiment response of ten genotypes to 200 mm nacl was tested to evaluate salt tolerant and salt sensitive genotypes. Download fulltext pdf download fulltext pdf download fulltext pdf. Tomato root and shoot responses to salt stress under different. Six different growth stages were exposed to the same salinity stress treatments using a completely randomized design, with three pots six plants per treatment. The fruit oxidative parameters h 2 o 2 and mda were determined, and the fruit mechanisms that confer protection to oxidative stress were investigated. Grafting tomato as a tool to improve salt tolerance. Effects of salinity stress at different growth stages on. Abstractthis study investigated the effects of salinity stress at different growth stages on the growth, yield, fruit quality, and wateruse efficiency wue of tomato solanum lycopersicum plants cultivated under soilless conditions. Two tomato isopentenyltransferases, slipt3 and slipt4, were. Salt stress response in tomato beyond the salinity tolerance threshold. Effect of salt stress on growth and chlorophyll content of some cultivated cotton varieties grown in syria.
Alternatively, the dynamic process of salt accumulation into the shoot relative to the shoot biomass has also been considered as a tolerance index. Crop salt tolerance is generally assessed as the relative yield response to increasing root zone salinity, expressed as soil ec e or irrigation water ec w electrical conductivity. Salinity acclimation ameliorates salt stress in tomato solanum lycopersicum l. Effect of salinity on tomato lycopersicon esculentum mill. We wanted to determine how salt stress would affect the wound response in tomato plants.
Salt stress was chosen because it is an abiotic stress that is initially perceived in the roots, in contrast to. In present study, germination rate, speed of germination, dry weight ratio. Salt stress was chosen because it is an abiotic stress that is initially perceived in the roots, in contrast to wounding that is inflicted on the leaves. Salt stress induction of proteinase inhibitor ii inh ii accumulation we wanted to determine how salt stress would affect the wound response in tomato plants. The isolate ej01 conferred increases in fresh weight, dry weight, and plant height of tomato and arabidopsis under both normal and high salinity conditions. Department of horticulture, the pennsylvania state university, 103 tyson building, university park, pa 16802, usa. Selection of salt tolerant rootstocks though screening of available commercial and wild relatives of tomato under salt stress conditions is a. In addition, overexpression of slmyb102 significantly decreased the electrolyte leakage and mda content in tomato leaves under salt stress fig. Response of tomato cultivars to salinity springerlink. Due to its role in absorbing water and nutrients, the root is the foremost part of the plant to encounter soil salinity. However, the underlying mechanisms of ck metabolism and signaling.